Following engagement with the Fc region of a relevant antibody bound to a target disease cell, Fc receptors transduce intracellular signals within the effector cell resulting in elimination of the target cell. These primary cell-based assays are highly variable as a result of donor differences and the requirement for cell culture and expansion.
The ADCC Reporter Bioassay provides a biologically relevant and specific MOA-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays. Specifically, primary donor PBMC or NK cells are replaced with a Jurkat cell stably expressing human FcγRIIIa (either the high-affinity V158 or low-affinity F158 receptor) and NFAT-induced luciferase. Importantly, the ADCC Reporter Bioassay demonstrates rank ordering of antibody activity equivalent to classic LDH release ADCC bioassays. The bioassay also can be used to quantify effects of antibody glycosylation on Fc effector function.
注意事项
ADCC Bioassay Effector Cells, Propagation Model, allows propagation and banking of the ADCC Bioassay Effector Cells. Bio-Glo™ Luciferase Assay System is the required reagent for use with ADCC Bioassay Effector Cells, Propagation Model. Product includes two vials at 2 × 107 cells/ml and 0.65ml/vial. One vial should be thawed, propagated and cells frozen to create a cell bank. The remaining vial should be reserved as backup.