The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods.

特点 - 优点

• High Sensitivity: Detect picogram levels of protein with minimal background.

• Save Time: No optimization required; you can switch from other entry-level ECL substrates.

应用

• Western blotting.

• Dot blotting.

储存条件

Store at 2–8°C.

Figure 1. Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.

The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng) as quantitated via ELISA. Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with ECL Western Blotting Substrate(Cat.# W1015) and with 1-minute exposure.