GoTaq® Probe qPCR Master Mix 2ml A6101
GoTaq® Probe qPCR Master Mix 10ml A6102
GoTaq® Probe 2-Step RT-qPCR System 2ml A6110
GoTaq® Probe 1-Step RT-qPCR System 2ml A6120
The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.
The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.
特点 - 优点
Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons.
Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.
应用
注意事项
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| Number of Reactions |
| Product Cat.# | Size | 50µl reactions | 20µl reactions | 10µl reactions |
| A6101 | 2ml | 80 reactions | 200 reactions | 400 reactions |
| A6102 | 10ml | 400 reactions | 1,000 reactions | 2,000 reactions |
| A6110 | 2ml | 50 × 20µl RT reactions and
80 × 50µl qPCR reactions | 50 × 20µl RT reactions and
200 × 20µl qPCR reactions | 50 × 20µl RT reactions and
400 × 10µl qPCR reactions |
| A6120 | 2ml | 80 reactions | 200 reactions | 400 reactions |
Figure 1. Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.
A series of fivefold serial dilutions of human pancreas cDNA was used to measure GAPDH gene expression using the GoTaq® Probe 2-Step RT-qPCR System and a competing product (Vendor L). The GoTaq® Probe 2-Step RT-qPCR System shows earlier Cq values (>0.2) for all samples. Two microliters of human pancreas RNA was reverse transcribed using the GoScript™ Reverse Transcriptase supplied in the kit followed by qPCR with GoTaq® Probe qPCR Master Mix.