The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.
特点 - 优点
Experience Minimal Binding: Highly specific to dsDNA; minimal binding to ssDNA, RNA, protein and interfering compounds.
Increase Your Sensitivity: Significantly increased sensitivity compared to absorbance at 260nm (NanoDrop® spectrophotometer) for low-concentration samples. Performs better or equal to PicoGreen® dye and can detect as little as 50pg/ml.
Set Up Quickly and Easily: System includes all required reagents to quickly set up and quantitate dsDNA.
Use with Promega Instruments: Pre-optimized on both the Quantus™ Fluorometer and GloMax®-Multi+ Instrument.
Use for Next-Gen Sequencing: Successfully used in several Next-Gen Sequencing systems, including Illumina (HiSeq/miSeq), Roche (454) and LifeTech (ion Torrent and ion Proton) systems.
应用
注意事项
Each QuantiFluor® dsDNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl.
Figure 1. Sample standard curve using the high-concentration standard curve 96-well-plate format in Section 3.A of the Technical Manual.
The final concentration of the standard in the 96-well plate 200μl assay volume is listed in Table 3 of the Technical Manual. Inset shows an enlargement of the results obtained with 0–100ng/ml DNA.
Figure 2. Sample standard curve using the QuantiFluor® dsDNA Dye and a competitor dye.
Please see the Technical Manual, Figure 2, for more details.