特点 - 优点
Increase Your Sensitivity: Significantly increased sensitivity compared to absorbance results on the NanoDrop® spectrophotometer, allowing you to quantitate low-concentration samples with confidence.
Save Precious Sample for Downstream Assays: Less template RNA required than for quantification by spectrophotometry.
Experience Flexible Instrument Compatibility: Use easily on both the QuantiFluor®-ST Fluorometer and GloMax®-Multi Instrument. This system also can be used on any fluorescent instrument with appropriate optical channels.
Remain Cost-Effective: Value priced, robust option for RNA quantitation.
Use with Promega Instruments: Pre-optimized on both the Quantus™ Fluorometer and GloMax®-Multi+ Instrument.
应用
注意事项
Each QuantiFluor® RNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl.
Figure 1. Standard curves using the QuantiFluor® RNA Dye and a competitor dye.
The standard curves were generated using RNA Standard in a 96-well format and 200µl total volume as described in Section 5 of the Technical Manual. The standard curve RNA amounts are 2ng, 10ng, 20ng, 50ng, 100ng, 200ng and 500ng per well. Fluorescence was measured using the GloMax®-Multi+ Detection System. The fluorescence values shown were blank-subtracted. Under these conditions, the dynamic range for the QuantiFluor® RNA Dye is approximately 2–500ng per well (in 200µl total volume), and the QuantiFluor® RNA Dye limit of detection is approximately 100pg per well.