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FGFR3 (K650E) Kinase Enzyme System
产品名称:

FGFR3 (K650E) Kinase Enzyme System

上市日期: 2017-01-25

 Recombinant human FGFR3 (K650E) (397–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Fibroblast growth factor receptor 3 (FGFR3) is part of a family of fibroblast growth factor receptors that share similar structure and function.

产品概述

FGFR3 (K650E) Kinase Enzyme System 10μg V5082

FGFR3 (K650E) Kinase Enzyme System 1mg V6598

ADP-Glo™ Kinase Assay + FGFR3 (K650E) Kinase Enzyme System 1 each V5083

 

FGFR3 plays a role in several important cellular processes, including regulation of cell growth and division, determination of cell fate, formation of blood vessels, wound healing and embryo development (1). FGFR3 is involved in the development and maintenance of bone and brain tissue. Mutations in FGFR3 have been implicated in causing bladder cancer, cancer of white blood cells (multiple myeloma) and cervical cancer (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

  • Kinase: FGFR3 (K650E), 10μg (Human, recombinant; amino acids 397–end). MW: ~73kDa.

  • Substrate: Poly (Ala6, Glu2, Lys5, Tyr1) (AAAAAAEEKKKKKY).

  • Other: Reaction Buffer, MnCl2, DTT.

FGFR3 (K650E) NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

特点 - 优点

  • Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.

  • Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.

  • Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

注意事项

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

储存条件

Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.

 

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