试剂作用温和，毒性低，使基于转染的实验中的细胞处于健康和新陈代谢活跃的状态。转染操作简单快速，无需在使用前去除血清或培养基。加入转染试剂：DNA混合物后无需洗涤或更换培养基。ViaFect™ Transfection Reagent拥有强大优越的性能，使您的实验更简单。
特点 - 优点
让您的实验拥有最佳的细胞模型：ViaFect™ Transfection Reagent适用于各种类型的细胞系，包括贴壁细胞、悬浮细胞和干细胞来源的细胞。
转染效率更出色：ViaFect™ Transfection Reagent在多种细胞系中转染效率更高。
使细胞保持健康状态：试剂毒性低，能够在转染过程中保持细胞的生物学和细胞代谢，从而更准确的呈现细胞模型的生物学状态。
操作步骤简单：简单易用的操作步骤，无需复杂优化。
应用
报告基因转染
细胞信号通路分析
肿瘤研究
干细胞研究
蛋白生产
Figure 1. Overview of adherent cell transfection protocol for a 96-well plate using ViaFect™ Transfection Reagent.
Figure 2. ViaFect™ Transfection Reagent enables an assay for cytokine signaling in a hematopoietic cell model.
TF-1 suspension cells were transiently transfected with pGL4.32[luc2P/NF-κB-RE/Hygro] Vector, an NF-κB response element luciferase reporter, using either ViaFect™ Transfection Reagent or Amaxa Nucleofector® II (electroporation). The following day cells were stimulated with TNFα for 6 hours, and the response was measured with Bio-Glo™ Luciferase Reagent
Figure 3. Directly assay HIF1A protein stability in iPS-derived cardiomyocytes.
iCell® Cardiomyocytes (Cellular Dynamics) were transfected with ViaFect™ Transfection Reagent using pNLF1-HIF1A [CMV/neo] Vector, a HIF1A-NanoLuc® luciferase fusion reporter. Twenty-four hours post-transfection cells were treated as indicated with hypoxia mimetics for 3 hours, and the amount of HIF1A-NanoLuc protein was detected using Nano-Glo® Luciferase Assay Reagent.
Figure 4. Assay for hypoxia transcriptional response in iPS-derived cardiomyocytes.
iCell® Cardiomyocytes (Cellular Dynamics) were transfected with ViaFect™ Transfection Reagent using the pGL4.42[luc2P/HRE/Hygro] Vector, a hypoxia response element luciferase reporter. Twenty-four hours post-transfection, cells were treated with hypoxia mimetics as indicated for 6 hours followed by luciferase detection using ONE-Glo™ Luciferase Assay System.
Figure 5. Cell lines successfully transfected using the ViaFect™ Transfection Reagent.