CD4+ Central Memory T Cell Isolation Kit, human130-094-302
产品说明
Central memory CD4+ T cells are isolated based on the expression of CD197 (CCR7) and the absence of CD45RA on these cells.
Detailed separation procedure
For the first separation, non-target cells are labeled with a cocktail of biotin-conjugated antibodies against CD8, CD14, CD15, CD16, CD19, CD25, CD36, CD45RA, CD56, CD123, TCRγ/δ, and CD235a (Glycophorin A) and Anti-Biotin MicroBeads to deplete non-CD4+ T cells, naive CD4+ T cells and non T cells. For the second separation, the flow-through fraction of the first separation is labeled with CD197 (CCR7)-PE and Anti-PE MicroBeads for positive selection of central memory CD4+ T cells.
Columns
For the first magnetic separation (depletion): LS or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
相关图片
Isolation of CD4+ central memory T cells from human PBMCs using the CD4+ Central Memory T Cell Isolation Kit, an LS and an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were stained with CD4-APC and CD45RA-FITC. Cells were already labeled with CD197 (CCR7)-PE during the isolation procedure.
PBMCs before separation
Pre-enriched CD4+ memory T cells
Isolated CD4+ central memory T cells