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CD4+ T Cell Isolation Kit, non-human primate130-092-144
产品名称:

CD4+ T Cell Isolation Kit, non-human primate130-092-144

上市日期: 2017-01-11

产品简介

The CD4+ T Cell Isolation Kit was developed for the isolation of untouched CD4+ T cells from rhesus monkey PBMCs.

产品概述

CD4+ T Cell Isolation Kit, non-human primate130-092-144

产品说明

Background information

Non-CD4+ T cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Highly pure untouched CD4+ T cells are obtained by depletion of the magnetically labeled cells.

Untouched CD4+ T cells may also be used for further isolation of specific T cell subsets. The CD4+CD25+ Regulatory T Cell Isolation Kit is recommended for most convenient separation of CD4+CD25+ regulatory T cells. Furthermore, the CD4+ T Cell Isolation Kit can be used in combination with CD95-PE and Anti-PE MicroBeads to isolate naive CD4+ T cells from non-human primate PBMCs. A special protocol for this application is available.

Downstream applications

T cells isolated with the CD4+ T Cell Isolation Kit are used when direct labeling of T cell surface molecules could interfere with downstrem applications, e.g., in studies on T helper cell activation.

Columns

MS, LS, or autoMACS® Columns.

相关图片

Isolation of untouched CD4+ T cells from rhesus monkey PBMCs using the CD4+ T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells are fluorescently stained with CD45-FITC, CD4-PE and CD3-APC. Plots show viable CD45+ leukocytes.
PBMCs before separation
Isolated CD4+ T cells
CD4+ T Cell Isolation Kit, non-human primate130-092-144CD4+ T Cell Isolation Kit, non-human primate130-092-144



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