The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.
The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single-step real-time amplification reaction.
The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.
特点 - 优点
Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments.
Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.
应用
Gene expression analysis.
Genotyping.
Detection of sequence variants.
Sensitive sequence detection and quantification.
注意事项
|
| Number of Reactions |
| Product Cat.# | Size | 50µl reactions | 20µl reactions | 10µl reactions |
| A6001 | 5ml | 200 reactions | 500 reactions | 1,000 reactions |
| A6002 | 25ml | 1,000 reactions | 2,500 reactions | 5,000 reactions |
| A6010 | 5ml | 50 × 20µl RT reactions and
200 × 50µl qPCR reactions | 50 × 20µl RT reactions and
500 × 20µl qPCR reactions | 50 × 20µl RT reactions and
1,000 × 10µl qPCR reactions |
| A6020 | 5ml | 200 reactions | 500 reactions | 1,000 reactions |