CD303 (BDCA-2)-FITC, human (clone: AC144)130-097-927
产品说明
CD303 (BDCA-2) antibodies have been used, for example, to identify, characterize, and enumerate plasmacytoid dendritic cells in whole blood of healthy and HIV-infected individuals, and for analyzing the role of DC-SIGN in HIV infection and transmission.7,11Furthermore, CD303 (BDCA-2) antibodies were also used for immunohistochemical staining, for example to identify plasmacytoid dendritic cells in tissue sections from patients with different inflammatory skin diseases.5,12,13
Clone AC144 recognizes the the CD303 (BDCA-2) antigen1,3 which is expressed on human plasmacytoid dendritic cells in blood, lymphoid (e.g. tonsils and bone marrow) and non-lymphoid tissue.3,6 Specific expression allows direct identification of plasmacytoid dendritic cells using just one marker.1–11 CD303 (BDCA-2)+ plasmacytoid dendritic cells in blood and bone marrow are CD11c–, CD123high, CD4+, Lin–, CD45RA+, CD304 (BDCA-4/Neuropilin-1)+, CD141 (BDCA-3)low, CD1c (BDCA-1)–, CD14–, and CD2–. They express neither myeloid lineage markers (CD13, CD33) nor Fc receptors (CD32, CD64, FcRI).1,6 CD303 (BDCA-2) is strongly expressed on freshly isolated plasmacytoid dendritic cells but down-regulated within 48 hours of culturing.1 Unlike CD304 (BDCA-4/Neuropilin-1), CD303 (BDCA-2) is not detectable onex vivo generated monocyte-derived or hematopoietic precursor cell-derived CD1a+ dendritic cells.1,9
The CD303 (BDCA-2) antigen is a novel type II transmembrane C-type lectin.3Remarkably, plasmacytoid dendritic cells can take up ligands via CD303 (BDCA-2), then process and present the ligands to T cells.1,3 Unlike binding of antibodies to CD304 (BDCA-4), binding of antibodies to CD303 (BDCA-2) inhibits type I IFN production, which is induced in plasmacytoid dendritic cells by, for example, the influenza virus.3,6
Alternative names
CLEC4C, BDCA-2, CLECSF11, CLECSF7, DLEC, HECL, PRO34150
Clone and isotype
| Clone | Isotype |
|---|
| AC144 | mouse IgG1κ |
特点
Unique antibody for DC research
Get accurate PDC counts in whole blood, bone marrow, and tissue sections
相关图片
Human peripheral blood mononuclear cells (PBMCs) were stained with CD303 (BDCA-2), CD123 and CD45 antibodies and analyzed by flow cytometry. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. A pregate of CD45+ cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems.
PBMCs from cynomolgus monkey (Macaca fascicularis) were stained with CD303 (BDCA-2)-APC and Anti-HLA-DR-FITC and analyzed by flow cytometry.
