Anti-IFN-γ-FITC, human (clone: 45-15)130-097-936
产品说明
IFNg, IFG, IFI
Clone and isotype
| Clone | Isotype |
|---|
| 45-15 | mouse IgG1κ |
Cross reactivity
The antibody is tested to cross-react with
Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)
相关图片
Human peripheral blood mononuclear cells (PBMCs), either unstimulated (left images) or stimulated with SEB for six hours. After two hours, brefeldin A was added for stimulated and unstimulated cells. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies as well as with CD4 (Vit4) antibodies and CD69 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. CD4+ lymphocets were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
Rhesus monkey PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ-PE. Cells were analyzed by flow cytometry. Plots show CD4+ lymphocytes gated according to CD4-APC staining.
Unstimulated control
Cynomolgus monkey PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ-PE. Cells were analyzed by flow cytometry.
Stimulated sample
Unstimulated control
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