Human CD40-Ligand, premium grade130-096-711
产品说明
Background information
CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4+ T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis.
Biological activity
Proliferation of CD19+ B cells
premium grade: ≥ 4×103 U/mg
Quality description
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
相关图片
Human CD40-Ligand activity assay: The specific activity is determined by proliferation assay using enriched CD19+ B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).
Human CD40-Ligand activity assay: The specific activity is determined byactivation assay using enriched CD19+ B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).
SDS-PAGE of Human CD40-ligand, premium gradeunder reduced (R) and non-reduced (NR) conditions.
Human CD40-Ligand biological activity. Activity of Human CD40-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).
Mass spectrometry analysis (ESI-MS) of Human CD40-Ligand, premium grade. The major peak corresponds to the calculated molecular mass of 23034 Da. The minor peaks correspond to gluconoylation derivatives as described in Geoghegan, K. F. et al. (1999).