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型号:
时间: 2017 - 01 - 11
CD4 MicroBeads, rat130-090-319产品说明Background informationCD4 is expressed on most thymocytes, T helper cells, CD4+ regulatory T cells, as well as on a subset of dendritic cells and, at a lower level, on macrophages and monocytes.Downstream applicationsT cells isolated with CD4 MicroBeads can be used for in vitro1 analysis, for example, of cytokine expression or antigen specificity. Functionality of the isolated cells has been shown by several adoptive transfer experiments.2–4 Transfer of isolated...
型号:
时间: 2017 - 01 - 11
CD4 MultiSort Kit, human130-055-101产品说明Detailed separation procedureThe CD4+ cells are isolated with CD4 MultiSort MicroBeads and the magnetic particles are then enzymatically released. The selected CD4+ T helper cells can again be magnetically labeled and sorted for a second antigen.Downstream applicationsThe CD4 MultiSort Kit can be used to isolate specific CD4+ T cell subsets. The selected CD4+ T helper cell population can be sorted according to a second marker of interest, e.g., CD45RA1,2,3,...
型号:
时间: 2017 - 01 - 11
CD4+ Central Memory T Cell Isolation Kit, human130-094-302产品说明Central memory CD4+ T cells are isolated based on the expression of CD197 (CCR7) and the absence of CD45RA on these cells.Detailed separation procedureFor the first separation, non-target cells are labeled with a cocktail of biotin-conjugated antibodies against CD8, CD14, CD15, CD16, CD19, CD25, CD36, CD45RA, CD56, CD123, TCRγ/δ, and CD235a (Glycophorin A) and Anti-Biotin MicroBeads to deplete non-CD4+ T cells, naive CD4+ T cells and ...
型号:
时间: 2017 - 01 - 11
CD4+ Effector Memory T Cell Isolation Kit, human130-094-125产品说明Detailed separation procedureFor magnetic depletion of non-target cells, PBMCs are incubated with a cocktail of biotinylated antibodies against CD45RA, CD8, CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, Anti-TCRγ/δ, and CD235a (glycophorin A) as well as APC-conjugated CD197 (CCR7) antibodies. In a second step, all antibody-labeled cells are labeled with MicroBead Cocktail for depletion. The CD4+Effector Memory T Cell Isolation Kit...
型号:
时间: 2017 - 01 - 11
CD4+ Recent Thymic Emigrant Isolation Kit, human130-094-299产品说明Background informationRTEs are a subpopulation of naive T cells characterized by their content of T cell receptor excision circles (TRECs).CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) has been described as a marker to identify the CD4+ RTEs among the naive CD4+ T cell population, as its expression correlates with the TREC content of CD4+ T cells1. CD4+ T cells lacking CD31 expression harbor little or no TRECs, sugges...
型号:
时间: 2017 - 01 - 11
CD4+ T Cell Isolation Kit, non-human primate130-092-144产品说明Background informationNon-CD4+ T cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Highly pure untouched CD4+ T cells are obtained by depletion of the magnetically labeled cells.Untouched CD4+ T cells may also be used for further isolation of specific T cell subsets. The CD4+CD25+ Regulatory T Cell Isolation Kit is recommended for most convenient separation of CD4+CD...
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