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型号:
时间: 2017 - 02 - 15
The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods.特点 - 优点High Sensitivity: Detect picogram levels of protein with minimal background.Save Time: No optimization required; you can switch from other entry-level ECL substrates.应用Western blotting.Dot blotting.储存条件Store at 2–8°C.Figure 1. Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293...
产品名称: Blocking Agents
型号:
时间: 2017 - 02 - 15
Blot-Qualified BSA 10g W3841Tween® 20 2.5ml 100% W3831Tween® 20 is a nonionic detergent used as a buffer component for immunoscreening in the ProtoBlot® Systems. In addition to blocking agents such as BSA, which saturate excess sites of antibody binding on membranes, this detergent acts in solution to dissociate nonspecific interactions with an antibody probe.
型号:
时间: 2017 - 02 - 15
另外,也可以用Canine Pancreatic Microsomal Membranes ( 犬胰微粒体膜) 结合TNT® Lysate Systems(TNT® 裂解物系统) 对合适的DNA 模板进行转录/ 翻译反应,从而研究加工和糖基化过程。为了性能稳定、翻译反应中的抑制作用最小、本底最低,制备微粒体时去除了污染的膜片段以及内源性的、结合在膜上的核糖体和mRNA。使用两种不同的对照mRNA 模板,对膜制备物的信号肽酶和核心糖基化活性进行了检测。这两种对照mRNA 是E. coli的β- 内酰胺酶( 或氨苄青霉素抗性基因产物) 前体,以及啤酒酵母(S.cerevisiae ) 的α- 配合因子( 或α- 因子基因产物) 前体。从含有编码大肠杆菌β- 内酰胺酶( 氨苄青霉素抗性基因产物)前体的基因序列的质粒上,使用SP6 RNA 聚合酶可转录得到对照信号序列mRNA( 大肠杆菌β- 内酰胺酶)。合成的RNA不含有帽类似物。该对照mRNA 用于检测信号肽酶活性,并与Canine Pancreatic Microsomal Membranes System ...
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